Hi Mohammed,

if you want to segment regions of a certain temperature range, the color (hue) is the relevant feature in your image. You can realize the color segmentation by doing a HSV (hue/saturation/value) transform first, and then do a thresholding with the hue channel, thus segmenting pixels that are in the required hue range.

Good luck and best regards,

Ralf

Hello,

You can refer these papers. Might be helpful.

Good luck.

Thank you guys for replying I appreciate 

Hi Mohammed , please check this link and i can provide you the source code as well if do you want.

http://www.sciencedirect.com/science/article/pii/S0925231216304829

 Rashid Mehmood thank you so much 

Hi Mohammed,

please check this Master’s Thesis:

Wretman, David (2006) Finding Regions of Interest in a Decision Support System for Analysis of Infrared Images

at the link: 

http://www.nada.kth.se/utbildning/grukth/exjobb/rapportlistor/2006/rapporter06/wretman_david_06135.pdf

In addition, some information you can find in this book: Rajendra Acharya U,Y. K. Eddie Ng, Jasjit S. Suri. Image Modeling of the Human Eye. Artech House, 2008.