in the event that I am interested in introducing a mixed microbial inocula into the GUT of a test animal to study the pattern of colonization of the GUT and probiotic potential of GUT microbiota.
Are these pathogens of the said test animal? Ingestion is one way.
The organism can be introduced along with an amount of feed the animal will consume in one meal (to avoid loss). Humane and simple i suppose
There can be two ways : one by mixing the organisms with diet, but maintaining aseptic conditions as well as assurance of the organism being ingested will be low.Feeding directly with a known quantity( preferably in terms of CFU) will be the better one.
The main problem is identification of the organisms introduced. The inoculum need to have a specific genetic marker preferably more than one. A chromosomal marker to follow the growth of organism in situ is essential. A marker on a damaged phage or plasmid has been tried to follow the clearance of the original inoculum.
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