What is the most efficient mouse brain weight for RNA extraction for NGS?

02 January 2021 1 8K Report

I have collected the brains and they are Fresh Frozen into PFC, Mid Brain and Brain Stem. I want to extract the amygdala and hypothalamus.

Trieu-Duc Vu

Hi,

In my case, I used Invitrogen Pure Link RNA Mini Kit and the amount of brain sample I used is 0.03g.

Hope it helps

Badges
Science topic

More Jeannie Devereaux's questions See All

Should I spike mice brain samples for HPLC?

I am about to run mice brain samples in the HPLC. I have optimised the standards however some methods are spiking their samples. Why do I need to do this?

11 April 2021 1,129 4 View

Lc/ms hplc?

I have mice brain tissue to perform LC/MS for Tryptophan Metabolites. I have also been told to look for inflammatory cytokines. Does anyone have experience with this or recommendations?

05 July 2020 6,837 5 View

Where can I get the ptfe-coated blanket used for the decal method for catalyst layer transfer-printing, for Polymer electrolyte membrane fuel cell?

When I am reading papers related to the fabrication process, only ptfe-coated blanket will be mentioned without any details about it, e.g. its production company. Anyone who is familiar with this...

05 August 2014 6,303 0 View

How can I do particles analysis for the given picture?

For the attached picture, I want to do particle distribution analysis on the black part. I used the particles analyzer in ImageJ, however, the result is weird, only 93 particles was counted. I...

19 June 2014 4,466 16 View

Microscopy magnification?

We know that the magnification of the specimen from the eyepiece is multiplying the magnification of objective lense by the magnification of ocular lens. If the microscope is connected to a...

17 September 2013 5,302 12 View

Can I use ethanol to clean up the slides?

I need to add some colloidal suspension solution on glass slide, can I just use ethanol to cleanse the slides? I read some paper that they are using Piranha solution to remove organic matter on...

27 August 2013 2,158 31 View

How does the electro-mechanical effect come about?

Take the ionic polymer-metal composites (IPMCs) as a example. When being exposed to electrical field, an IPMC strip will experience shape-deformation due to the internal mechanical force induced...

30 April 2013 4,777 7 View

Imaging of catalytic ink

Now I want to capture the surface morphological change of the catalyst ink applied on a substrate, intend to use a CCD camera with maximum resolution of mm. However, the catalytic ink is black, it...

25 April 2013 5,521 2 View

H.S. Art students in Self-Quarantine without supplies

As Spring Break is coming to an end and we "virtually" go back to school Monday entering into week 4 of Distance Learning, just how can the problem of my Title 1 students without Art materials to...

01 January 1970 824 0 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Can telekinesis, telepathy, and prediction be achieved through science?

Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...

28 July 2024 7,522 0 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View

How to add viscosity into a hyperelastic material such as Mooney-Rivlin rubber in LS-DYNA?

Hi, I am working on a head FE model and want to try different material types on brain. I read some papers that use Mooney-Rivlin rubber model and wondering if i can add viscosity into it. Is it...

24 July 2024 5,908 0 View