I am currently studying gene editing approaches and want to understand the best strategies to confirm a successful gene knock-in after CRISPR-Cas9 editing in mammalian cells. Since CRISPR often results in mixed outcomes (precise knock-ins, indels, or random integrations), I would like to know which verification methods are considered the most reliable and widely used. Some approaches I am aware of include PCR-based genotyping, sequencing, and protein-level assays, but I am not sure which combination provides the most accurate and efficient validation.

Could anyone suggest the standard or most effective workflow for confirming a precise knock-in event in mammalian systems?