trying to make chitosan nps - need to make chitosan disolve in water first - i use acetic acid but it is not able to dissolve the chitosan 100%
Can anyone suggest me some other more efficient way.
PS thanks in advance.
chitosan is sparingly soluble in water, except some of its derivative such as chitosan hydrochloride (available at sigma aldrich). Up to 2g of chitosan can be dissolved in 100 ml of acetic acid (1-2 % prepared with distilled or deionized water) to give a highly viscous solution. An homogenous mixture will require using a magnetic stirrer.
Try out with colloidal chitosan. prepare it by treating it with orthophosphoric acid (10g in 100 ml of 85% o-phosphoric acid). keep at 5 degree for 24 H. treat it with tap water and seperate the gelatinous wgite material by filtration using a filter paper. you will get clumps treat it with water till pH is 7.0. store in a closed container at 4 degree Celsius.
If you can take it through process that allows you to introduc replaceable hydroxyl groups after separation of its chains using say ionic liquids, then you might be able to get it maximally in solution while preserving its native state to a reasonable degree.
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