What is the most common culturable genus of bacteria from the marine environment?

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Number of microbial cells from marine coatal environment was 2 orders of magnitude less than expected. Is this just natural variance?

The number of microbial cells per mL from marine environments has been quoted as 1 x 10^6 cells. However water sampled from a marine coastal environmnet (popular beach, downstrean from a waste...

17 January 2017 5,536 3 View

Aligning sequences of bacteria did not aid identification. Use of one primer (forward) gave the results as using two primers (forward & reverse). Why?

From a sample of 10 marine bacteria I was able to succesfully identify these bacteria down to genus level using only one primer (forward). II then sent for the reverse primer to be sequenced. When...

11 December 2016 3,920 5 View

Aligning sequences of bacteria did not aid identification. Use of one primer (forward) gave the results as using two primers (forward & reverse). Why?

From a sample of 10 marine bacteria I was able to succesfully identify these bacteria down to genus level using only one primer (forward). II then sent for the reverse primer to be sequenced. When...

10 December 2016 4,986 4 View

How do I make a saline solution of a fixed concentration of a fixed pH value using only sodium and sodium bicarbonate?

A 3.5% saline solution prepared with water and sodium has a pH of 6.38. I want to make a solution of 3.5% salinity with a pH of 8.1 how do i do this using only sodium, and sodium bicarbonate? I...

16 October 2016 1,825 3 View

How to make serial dilutions of sea water whilst maintaining the pH and salinity of the seawater?

When distilled water is adjusted to suit the ocean's salonity the pH of the sample drops to 6.3. The addition of buffer to adjust solution will lower salinity. How do you achieve both desired pH...

10 October 2016 7,778 5 View

How do I make a saline solution of a fixed concentration of a fixed pH value using only sodium and sodium bicarbonate?

A 3.5% saline solution prepared with water and sodium has a pH of 6.38. I want to make a solution of 3.5% salinity with a pH of 8.1 how do i do this using only sodium, and sodium bicarbonate? I...

09 October 2016 3,128 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

What role do microorganisms play in nutrient cycling and maintaining soil health in nature and role of microorganisms in the cycling of bio elements?

31 July 2024 3,965 0 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

How do pollutants in soil affect soil microorganisms and role do microorganisms play in nutrient cycling and soil fertility?

31 July 2024 368 2 View

What's biological importance of bacteria &role of microbes improve crop productivity &soil health &role of bacteria as decomposers incycling nutrient?

What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?

31 July 2024 3,479 5 View

Claudete Rodrigues Paula

well, I do the médium for the isolation to this bactéria with sea water ; for me this indication is ok , regards , claudete

Phil Geis

Not gammaproteobacterium, but consider Pelagibacter ubique

Sujith P.P

Mostly the members of Gammaproteobacteria and Firmicutes are culturable from the marine environment with lot of heterogeneity in the strains obtained depending upon the environment and type of growth medium employed in the experiments.

Pranab Bhattacharyya

Dear O'Reilly,

Firmicutes and Actinobacteria were also reported from the deep-sea sediments.