I want to know which medium is preferable for the isolation of thermophilic bacteria (50 c) from agricultural soil?
Nutrient media incubating over 50 degrees is appropriate and the use of serial dilutions is really effective to increase the isolation of less frequent environmental bacteria and / or avoid nutrient competition.
I recommend you as well the use of VL medium; I found really helpful the following publications: Janssen et al, 2002 (DOI: 10.1128/AEM.68.5.2391–2396.2002) and Schoenborn et al, 2004 (DOI: 10.1128/AEM.70.7.4363–4366.2004).
Regards
You can use any general media.e.g. Nutrient agar, blood agar and soy agar
You can isolate on nutrient agar. However you need to prepare thik layer of agar than normally used for isolation of mesophilic bacteria.
I would suggest to enrich the soil sample in nutrient broth by incubating it at 50 to 55 degrees and then spread that on nutrient agar (undiluted and serially diluted)
Put a small quantity of soil sample in the nutrient broth, since it may have many microbes and it may lead to unnecessary competition for nutrients and loss of the desired organism. though, the high temperature of incubation will help in selecting the microbes you are interested in.
Nutrient media incubating over 50 degrees is appropriate and the use of serial dilutions is really effective to increase the isolation of less frequent environmental bacteria and / or avoid nutrient competition.
I recommend you as well the use of VL medium; I found really helpful the following publications: Janssen et al, 2002 (DOI: 10.1128/AEM.68.5.2391–2396.2002) and Schoenborn et al, 2004 (DOI: 10.1128/AEM.70.7.4363–4366.2004).
Regards
Dear Walid,
You can use any media which are specific for bacteria either as broth or solid
are you looking out for specific organism or just thermophiles? medium will vary for each organism. If you want to know population in a sample then you will have to use different media for each system.
Otherwise just inoculate sample in any medium and grow it at 50.. u will get some colonies.. if your sample has any
you can use specific medium like : TT medium/ Thermus medium/ 162 medium
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