I am doing my research project at university Tarbiat Modares , and currently designing a bioreactor for biodegradation of petroleum hydrocarbons, it is somewhat continuous bioreactor.
the aim of the present work is The peroxidase-mediated biodegradation of petroleum hydrocarbons in a H2O2-induced using in-situ production of peroxidase
but i need some help how to choose bioreactor
Any prospective leads and suggestions would be highly appreciated.
Thank you.
Saeed Molaei
What type of microorganisms and treated samples do you have?
regards,
G
The use of control reactor to which no bio-simulator was added. In this system most the hydrocarbon degradation occurred within the first four weeks. For more details. https://www.hindawi.com
Please refer to this link:
https://www.wiley.com/en-us/Bioreactors%3A+Design%2C+Operation+and+Novel+Applications-p-9783527337682
Good luck.
In my opinion first you try for batch reactor and optimise you factors separately then compare using kinetic studies with those of tubular reactor and CSTR reactor( reaction rate, reactor volume and reusability of media used).
For more information refer to the given material,
Grzegorz Boczkaj : based on sequence analysis and phylogenetic investigaion, dominant bacterias are Pseudomonas and bacillus
As stated Faheem, you should start with batch reactor and after optimisation of conditions make a scale-up or work with continous reactors.
regards,
G
Dear Saeed,
Your question is hard to answer since there are many bioreactor configurations you might use for hydrocarbon biodegradation. From my point of view, it depends on the type of hydrocarbons and the environmental conditions required (e.g. aerobic, anaerobic, anoxic, etc.). If you are dealing with volatile hydrocarbons, the best configuration could be a biotrickling filter or biofilters. Alkanes, pneumatic systems such as bubble columns or airlifts could be a good option (not a good option when you also have volatile hydrocarbons due to stripping). Heavier fractions could be better treated in systems with mechanical agitation. This rises the question on which matrix do you have your hydrocarbons: soil, water, air?? All these characteristics will move the balance towards different reactor configurations. Some literature that could help you:
Alkanes
https://drive.google.com/file/d/0Bwr38oMUGS5TMlRwRXZjNFg1UFk/view
Volatile hydrocarbons
https://drive.google.com/file/d/0Bwr38oMUGS5TMVRXUTJldEhuVHM/view
https://drive.google.com/file/d/0Bwr38oMUGS5Tdk41NlZjSEtHVm8/view
All
https://drive.google.com/file/d/0Bwr38oMUGS5TUmlHRzdFYmRCQlU/view
The inoculum in all cases could be activated sludge form a wastewater treatment plant, or soil from a contaminated site.
I basolutely agree with the answer of Dr. Guillermo Quijano above.
Yourt question is to general without input information what should be treated (which hydrocarbons, concentration, treatment limits, ....). When your aim is "The peroxidase-mediated biodegradation of petroleum hydrocarbons in a H2O2-induced using in-situ production of peroxidase" you must have some information about this processes, the kinetics and production rate of perooxidase at least. It is not possíble to design any reactor without knowledge of the reactions running in the bioreactor, their rate, kinetics, reaction time, efficiency etc. etc.
I recommend you to think over what you want to do exactly at first.
Best regards
Vit
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