What is the minimum percentage of identity required by BLAST to determine new species in plant if I use ITS?
Declaring a new species takes more than ITS non-homology
https://www.iapt-taxon.org/nomen/main.php
But I dont believe there is a set threshold, it could only be interpreted in a distance matrix
Please explain about the IRF (Instrument Response Function)? How it is measured and why it is important?
23 January 2023 1,700 2 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View
Hello, in January, I successfully used BLAST to find a homologue of HDV in a TSA dataset from a lizard. However, when I recently attempted to search for that sequence again, I couldn't find it in...
02 June 2024 7,415 1 View
Hi everyone, I run PCR to checked presence of specific gene in Clostridium perfringens but I received 2 bands, one band showed product size expected and another one showed higher product size. I...
31 May 2024 645 1 View
I am simulating a tunnel under blast loading using conwep method in ABAQUS,I would like to know the analysis procedure and steps of analysis?
17 May 2024 9,403 1 View
I did BLAST of the two cellulolytic species and one lignolytic species of bacteria after 16S amplification using PCR. However, my results indicate that the lignolytic organism is 100% similar to...
15 May 2024 4,483 8 View
Dear Colleagues, I am seeking guidance on a specific issue regarding the output of a blast analysis. In the results, I frequently encounter multiple gene IDs that share a similar sequence, but are...
15 May 2024 7,415 2 View
We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...
25 April 2024 6,159 0 View
when I insert my primers in primer blast site and push the button "get primers" I just reach different errors and I don't know what the problem is!
22 April 2024 8,001 5 View
Hello Researchers & Professors, Limited research has been done on effect of high strain rate on concrete due to blast loading using machine learning techniques. According to study we want to...
22 April 2024 2,181 1 View