Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

I have a problem with sensible heat flux (H) iteration process in Sebal model?

In each iteration, I get the same value of H for different values of aerodynamic resistance and the temperature difference between two heights. Do the results make sense? Is it necessary to adjust...

07 February 2021 8,953 2 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

Why am I getting 2 bands for Gibson Cloning Vector PCR?

Hi, I designed Gibson primers (2 primers for fragment and 2 primers for vector) using snapgene. Then I ran PCR separately for Fragment and Vector. Fragment DNA was found to be the correct sized...

11 January 2021 9,500 2 View

Was there any pesticide disasters other than Bhopal and Kasaragood tragedy around the globe?

Pesticides become prime compo in agricultural activities to meet the growing demand of the human population. But indiscriminate usage and mishandling led to two disasters in India such as Bhopal...

11 January 2021 3,790 7 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

Can we mix elemental powders of 325 mesh using an agate mortar and pestle?

Elemental Powders like Titanium, vanadium, tungsten, zirconium, hafnium.

04 January 2021 7,357 1 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View

Which tropical country is the most easy for obtaining research permit (for biological research)?

Dear all, High beurocracy hihly complicate field ecolocial and evolutionary research nowadays. Nearly all tropical countries (maybe all?) requires research permit for performitng research and in...

04 February 2021 3,672 6 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View

Can R be used for SEO?

13 January 2021 1,920 1 View

Are the mice expected to develop obvious airway disease after HDM? Will they have signs of respiratory distress at any time during the study?

I am developing model of asthma in mice using HDM. I was asked this question in order to submit the a protocol for developing a disease in animals. Searching in the literature, I can not find any...

30 December 2020 6,455 3 View

Hi, How we can sanitize or prevent fungal growth outside the bags in the high humidity of the chamber?

Hi, How we can sanitize or prevent fungal growth outside the bags in the high humidity of the chamber?

28 December 2020 2,486 2 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

Santolina species, wax moth and bees?

Expect a savage answer and being reported to ResearchGate Admin if your answer clearly shows you did not read the whole question and you are scamming the points system by answering with an...

12 December 2020 4,413 7 View

What was scientist thinking about Bees communication before Karl von Frisch discovery?

In the 1920s, Karl von Frisch pointed out that bees use special dancing patterns. What scientist or people think about bees communication before its discovery.

22 November 2020 1,426 5 View

LIFE in SMART CITIES: Animal data versus Human data, complementarity or not?

One observation is that in any city (those I know) you have humans, of course, but you also have many animals. Statistics of humans start with population data (for instance demographics) and...

08 November 2020 4,127 6 View

Preserving mites in a mixture of ethanol and propylene glycol?

Ethanol and propylene glycol are both used to preserve mites, and each have their drawbacks (i.e., evaporation and specimen distortion). Some people add a small amount of glycerol to vials of...

08 October 2020 9,516 6 View