How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

Can Vibrio campbellii bioluminescence be obtained when cultured on Mueller-Hinton agar?

Need to check quorum sensing activity of Vibrio campbellii.

20 January 2021 570 5 View

Which identification method is the most suitable or identification of Vibrio spp.? Molecular or Biochemical?

I have some Vibrio isolates from Brackishwater fish and environment. I am unable to identify two of them unto species level. It is showing conflict between Biochemical identification and Molecular...

03 January 2021 691 7 View

Is there a way to automatically gather surface area from a 3D model based on color/texture?

I have been constructing 3D models of large corals and I was wondering if there is a software that I can use to gather the area of live tissue vs dead/disease? I am aware there is a way by tracing...

17 December 2020 6,991 1 View

Rapid color change in fishes?

I have noted dramatically change in color on the body of some marine fishes. For example, I saw a dramatically change from a "intense-yellow" color to "brown" color (normal color), just in one...

19 October 2020 2,717 6 View

Is there any current research being done on ocean species that can convert carbonic acid?

For a class I am looking at the effects of ocean acidification, and within this although I am finding that there are species that are both negatively (coral, oysters, etc.) affected and positively...

11 February 2020 4,212 2 View

Is there any current research being done on ocean species that can convert carbonic acid?

For a class I am looking at the effects of ocean acidification, and within this although I am finding that there are species that are both negatively (coral, oysters, etc.) affected and positively...

01 February 2020 349 2 View

Methods for removing tissue from coral for chlorophyll and protein isolation?

Hello all, I'm looking for alternative ways to process frozen coral samples to quantify chlorophyll and proteins that doesn't involve airbrushing the tissue from the corallite. I've found a few...

10 November 2019 9,416 3 View

How the intracellular protein provide immune protective effect for host?

In our research, the DNA vaccine encoding Hsp33 gene of Vibrio anguillarum was made and proved to confer immune protective effect for fish. however, the Hsp33 was belong to the intracellular...

08 September 2019 1,209 5 View