from my experience, microalgae need some time for adaptation to the new setting (culture medium, cultivation condition and cultivation mode). i usually put more than the initial seeding that i normally use which is 10%. it depence on the adaptation capacity of the microalgae to the PBR/open pond. last time, i used 30% of initial seeding to adapt spirulina in waste water. because it takes too long for the adaptation process. after the adaptation process, i used 10% of initial seeding for my original experiment. so, u can modify the initial seeding based on the adaptation process of the microalgae....
Depends on the configuration, but I usually use between 5-10%; takes a while to adjust, but it is not the reason to use more than that; well, this is my opnion.
well, 10% is enough but its very important to adapt the algae to the new condition + the seed must be very concentrated in term of the density
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