It depends on the kit, read the kit brochure

yes, it depends on the pol you are using!! check the pol you have order!

Hi Jameela,

I agree with Alaaeldin and Sabrina.

Warm regards,

Venk

very thanks my dear  Alaaeldin , Sabrina and venk

with best wishes 

You should follow the instructions of the manufacturer...

 I agree with Dr. Alaaeldin

Free magnesium concentration in isolated rabbit hearts subjected to high dose isoproterenol infusion: a 31P NMR study

I Mottet, J F Goudemant, M Francaux, R Demeure, X Sturbois

Article Free magnesium concentration in isolated rabbit hearts subje...

helo dear dr.

yes, it depends on the pol you are using!! check the pol you have order

helo dear dr.

yes, it depends on the pol you are using!! check the pol you have order

https://www.neb.com/.../guidelines-for-pcr-optimization-with-taq-dn...

Mg2+ complexes the single nucelotides in your pcr reaction, and only Mg/nucleotide-complexes are the substrate for the dna-polymerase. so, no Mg2+, no amplification

What is the function of MgCl2 in PCR?. Available from: https://www.researchgate.net/post/What_is_the_function_of_MgCl2_in_PCR/1 [accessed May 13, 2017].

hi. A low Mg2+ concentration requires more stringent base pairing in the annealing step

Optimal concentration depends on template, buffer, DNA and dNTPs (each has the potential to chelate magnesium)

Dr. Jameela,

Please have a look at this link for the answer to your query.

http://primerdigital.com/pcr.html

Good luck

Thanks , all my dear for your answers