What is the loading control for ER and Golgi membrane extract in western blotting?

More Purusottam Mohapatra's questions See All

I need MD simulation in 500 ns for some of my test compounds with PBSA, GBSA and PCA. If a researcher will help, we will provide authorship?

For my research, I need MD simulation of 500 ns with PCA. Please reply if you have facility. We will provide corresponding authorship. The article will be published in a high IF journal. My email-...

26 November 2023 6,926 0 View

How to increase the solubility of chlorophyll extracted from plants for bioimaging studies?

Please provide information related to any coating that can be done or any composites that can be synthesized.

09 October 2023 9,003 2 View

As we all know that Si 2p peak can be deconvoluted into two peaks such as Si 2p1/2 and Si 2p3/2. Which peak has higher binding energy and why ? ?

In the few literature, it was mentioned that the binding energy of Si 2p1/2 was higher than Si 2p3/2, while some authors made the just opposite statement about these peaks. I am so confused about...

22 June 2023 1,135 2 View

What is the JCPDS No and CCDC no for Ag-MOF-5?

Ag-MOF-5 synthesized from Terepthalic acid, Silver Nitrate and DMF. Need to know Chemical formula, Molecular weight and molecular structure of Ag-MOF-5. Thank you

21 May 2023 1,624 0 View

Can a composite show different dual phases (space group) in room temperature XRD and room temperature Neutron diffraction Reitveld refinement?

If possible kindly suggest some references.

05 February 2023 3,683 3 View

How to isolate fibroblast cells from hamster?

I isolated fibroblast cells from hamster by collagenase A digestion method. I used DMEM with extra L-glutamine as culture media. After 24hr there is no attachment of cells. The flask was coated...

28 November 2022 4,816 1 View

Which is the best technique- FACS or MACS for separation of dead cell from live cell population by using PI and SYTO9 fluorescence dye?

I want to separate dead cells from a bacterial population after treatment of any drug or antimicrobial agent. Which technique will be more efficient FACS or MACS if I use PI and SYTO9 dye ? Is...

15 August 2022 4,009 3 View

How to separate my metal microcage synthesised in ethylene glycol and water solvent ??

I tried to pellet it down at a maximum of 18K rpm, but it did not work. I heated the reaction mixture up to 200 C temp to evaporate the Ethylene Glycol(Including water) and to get the metal...

21 June 2022 6,740 0 View

Can anyone please help me with a protocol for in-vitro phosphorylation using CAMK2α ?

I want to phosphorylate my protein using CAMK2α. I ordered CAMK2α Kinase Enzyme System from promega...

06 June 2022 5,693 0 View

Is it possible to cryopreserve the Neonatal primary cardiomyocyte?

Neonatal cardiomyocyte is the sensitive primary cell line. Can a researcher cryopreserve the cell line for future experiment?

30 December 2021 4,160 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

Cristián Quintero Popular answer

Dear Purusottam Mohapatra

If you are looking for endogenous bona fide markers for ER and Golgi, I can suggest:

ER: Calreticulin, Calnexin, PDI,

Golgi: GM130, TGN46, Giantin, Mannosidase II

There are several good commercial antibodies for each of them, available and useful for WB and IF.

Good luck

Cristián Quintero