I got so many peaks and it also increases what is the interpretation for it.
Please check the attached file maybe it will be useful to you!!!!
This seems to be very much unusual. You should be the base line correction perfectly
What do you mean with "step-wise" absorption?
Which compound?
Which instrument?
How many peaks?
How much increase in what time period?
ReseaechGate asks from you to be specific so that you can be helped
It could be a vibronic progression but it is necessary to have further information (which compound, under which conditions, etc.) to be sure.
It seems to me like an instruments artefact. Absorption spectrometers usually have a filter wheel in the optical path, and each time the filter is switches, a step can occur in the spectrum. May be you can try to reduce the scan speed and increase the integration time for each data point.
Good Luck, Frank
Hope this is one of the few questioners who do respond to requests for more info on their problem: it's already 2 days without response
Instrument name JASCO UV Vis NIR
Model name V-670
Serial No. A056961154
Accessory USE-753
Accessory S/N A056961154
Cell length 10 mm
Photometric mode Abs
Measurement range 1100 - 200 nm
Data interval 1 nm
UV/Vis bandwidth 2.0 nm
NIR bandwidth 8.0 nm
Response Fast
Scan speed 1000 nm/min
Change source at 340 nm
Change grating at 850 nm
Light source D2/WI
Filter exchange Step
Correction Baseline
Thank you for the info
Remaining IMPORTANT questions for you to answer:
What is the absolute value of the absorption in log(Io/I)
You do not name the compound? or are you measuring the solvent
Is there a change in filters at the steps in your "spectrum"?
I think, its quite abnormal... do repeat again and confirm after calibrating the instruments with standard spectrum.
Mr. Anandhi,
Please find a short explanation according the type of the substances as attachment. Hope that it shall be useful for you
mrs Ivanova
You should have a look at the "spectrum" that the questioner provided; no remblance with a standard spectrum (BTW: a very nice illustrative summarising figure!, but molecules may have more vibronic structure)
His figure is as attachment on page 1 of this question
Mr. ten Brink,
When I have tried to open the shown file with OpenOffice there has not any fig. Most probably it should be better if Mr. Anandhi has attached the spectrum in*gif format. few additional comments about the nature of the analyte should be also very useful no doubt.
I could open it with WordStarter
You really should have a look because the absorption is large with a shape that in no way resembles a molecular specgtrum
Mr. ten Brink,
I definitely cannot assign the spectrum shown to real spectrum of chemical substance. There have artefacts, assuming that most probably the source of the instrument need to be changed. A xenon source is high recommended one.
Dr Ivanova
I fully agree with you. The absorption strength of this artefact is alarmingly high
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