I was doing some research on ABE fermentation using bacterium Clostridium acetobutylicum.Before fermentation, deactivated bacteria cultures are stored at -80 °C. The thawed cells are then inoculated into 12 mL synthetic medium containing glucose (30 g/L) and Yeast Extract (5 g/L) in 15 mL Hungate tubes (pre-cultures). Cells are then grown under an aerobic conditions for 48 h at 37 °C, thereafter they are transferred into fermentation bottles.
For flask cultures, a single colony is inoculated into a test tube containing 10 ml of clostridial growth medium and anaerobically cultured at 37°C until the OD600 reached 2.0. Capped flasks containing 150 ml clostridial growth medium supplemented with 60 g/liter glucose are inoculated with 5 ml of this culture and grown at 37°C. At 48 h after inoculation, the samples are taken from the flasks and used in further analysis.
So what role does inoculation play in ABE fermentation?
At -80C the cells have a very low metabolic activity with significant mortality, and hence you need to activate the same before you use it as a inoculum. Here only those cells which are not permanently damaged gets activated and others do not. Thereby you get a population of cells capable of carrying out the activity you have designed for.
How is this inoculum administered to the substrate? How much of each is required?
Which clostridium species gives the highest concentration of butanol from the broth?
- Badges
- Science topic
- Similar topics
- Philosophy
- Philosophy Of Science
- Reasoning