11 April 2023 1 10K Report

antigen and antibody mechanisms

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How to find peak period and significant wave height for a particular location in ocean?

I want to develop the figure like attached figure. How can I get, peak period and significant wave height at any given location.

13 July 2024 886 2 View

How can we reduce the porosity of tungsten oxide (WO3) thin films during DC sputtering deposition (target: W, power: 100 W, 75 W, and 50 W)?

We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...

01 July 2024 2,686 2 View

Dear All, I have to plot a wave data similar to the attached figure in matlab. Anyone please help me in this?

How to plot like attached figure in matlab?..

25 June 2024 7,220 4 View

How to calculate drug loading and Encapsulation efficiency ?

Even after using the formula i am not getting the answer can anyone help me with one example .

28 May 2024 4,503 1 View

Black dots in cell culture ?

We have been getting this black dots in our cell culture. But it is not affecting pH of media (no color change observed) until 4 or 5 days. Cells are attaining morphology within 24hrs but once the...

12 May 2024 9,284 4 View

Increasing nozzles height of drone spryaing system increases droplet size. What could be the reason behind it? ?

Increasing nozzles height of drone spryaing system increases droplet size. What could be the reason behind it? ?

03 March 2024 4,127 0 View

Which is the more accurate method to calculate LUMO value for a D-A system?

Which is the more accurate method to calculate LUMO value for a D-A system? Either it from absorption edge or from CV measurement on reduction side?

15 February 2024 5,635 1 View

How do I overcome or eliminate this noise problem during patch clamp recordings?

Hello everyone, I recently encountered a noise problem in my patch clamp experiments. I happened to observe a small current and a noise when the electrode holder was in the open circuit. The...

28 January 2024 927 3 View

How long does it take to culture single colonies from single suspension cell in a 96-well plate?

Hello! I have been culturing GM11994- B lymphocyte cells from Coriell Institute. They are suspension cells and like to grow in colonies. However, after genetic modifications, I sorted single cells...

09 January 2024 7,779 0 View

How to calculate composite cylinders vibrations?

Papers that compute natural frequencies of cylinders made of composite materials.

29 October 2023 6,669 0 View

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Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

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Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Do I need an antigen retrieval for a multiplex RNAscope experiment for adherent cells?

Hello! I planned to do an multiplex RNAscope fluorescent assay for cultured cells in a 96 well plate format according to the protocol provided by ACD:...

22 July 2024 3,334 0 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

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