Acetonitrile-water systems are commonly used for many oligosaccharides and monosaccharides. Depending on the polarity of the aglycone, you should try anything from 98% acetonitrile/2% water to 75% acetonitrile/25% water. Multiple ascents may also be useful. This entails running the solvent to the top of the plate, drying the plate, and running it again.  Be sure to dry all of the DMSO from the applied spot before running the plate; otherwise, it will cause streaking and poor resolution. Since DMSO is difficult to completely remove, you might consider other solvents.

Acetone-EAA and Acetone -Petether

Dear Prof. Cote,

Thank you very much for your answer. however, it is very difficult to remove DMSO from the TLC spot. In general, what method do you use to remove it. Can you suggest other solvents as substitute of DMSO. In my understanding water is also not good and moreover, all glycoside are not soluble in it.

Thank you again for your suggestions and help.

With best regards!

Dr. M.Khan

Dear Dr.  Khansolepatil,

Thank you for your answer.

With best regards!

Dr. M.Khan

I almost never use DMSO as a solvent, so my experience is limited. It may be helpful if you could dilute your samples with ethanol or water prior to spotting them on the plate, and spot the sample in incremental amounts, drying with a hair dryer in between each spotting. The best advice I can offer is just to try it, make sure the spot is as dry as possible, and see what happens.

Please refer to the Book entitled "Encyclopedia of Chromatography".

Try this solvent system with varying percentages

ethyl acetate: acetic acid: formic acid: water

You can use ethyl acetate: methanol: water (20:2.5:2.5) for mono and di glycoside. if you see more polar compounds than increase some more polarity by adding methanol and see what happens

Dear all greetings,

thank you very much for your answers.

with best rgards

Dr. M.Khan

In general, the mobile phase TLC for flavonoid glycosides is ethyl acetate - formic acid - glacial acetic acid - water (100:11:11:26). If the addition of methyl ethyl ketone (ethyl acetate-methyl ethyl ketone-formic acid-glacial aseta acid - water (50:30:7:3:10)

detection is by uv  light 

or hydrolise the glycosides and try for the aglycones is another best method for tlc. 

some more solvwent systems: may be useful for flavonoid glycosides tlc

n-BuOH–HOAc–H2O, 65:15:25

n-BuOH–HOAc–H2O, 3:1:1

EtOAc–MeOH–H2O, 50:3:10

EtOAc–MeOH–HCOOH–H2O, 50:2:3:6

EtOAc–EtOH–HCOOH–H2O, 100:11:11:26

EtOAc–HCOOH–H2O, 9:1:1

EtOAc–HCOOH–H2O, 6:1:1

EtOAc–HCOOH–H2O, 50:4:10

EtOAc–HCOOH–HOAc–H2O, 100:11:11:26

EtOAc–HCOOH–HOAc–H2O, 25:2:2:4

THF–toluene–HCOOH–H2O, 16:8:2:1

Chloroform –MeCOMe–HCOOH, 50:33:17

Chloroform –EtOAc–MeCOMe, 5:1:4

Chloroform –MeOH–H2O, 65:45:12

Chloroform –MeOH–H2O, 40:10:1

MeCOMe–butanone–HCOOH, 10:7:1

MeOH–butanone–H2O, 8:1:1

Dear Prof. Rao Knv  greetings,

Thank you very much for your answers.

With best regards

Dr. M.Khan