Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Reinforcement learning toolbox?

I am looking for Matlab Reinforcement learning Toolbox, Can anybody help me? I will b so thankful.

31 May 2020 3,767 3 View

Which module is better for parallelism numpy functions in Python?

Which you recommend?Numba,Joblib,Dask and etc.Why?

20 March 2020 4,529 4 View

Can anyone advise if there is any company that sells a specific antibody for the HLA DRB1*1501 allele?

Hi everyone, I would appreciate if someone can give me some advise regarding Can anyone advise if there is any company with a specific antibody against HLA DRB1*1501 allele (DR 15 allele HLA...

29 November 2019 1,923 2 View

In Rev manager with continuous data, what number do we have to put in the Mean part while we have two pre and posttest means in the main articles?

I am working with Rev manager 5.3, doing a meta-analysis on 8 studies that each compare 2 psychological treatments and found no difference between them.

25 September 2019 6,804 1 View

Would you remind me some universities or professors for study a phd about food science industry for astronauts?

thank you i am narges heidari.

23 November 2018 3,300 3 View

Have complex values of phonon frequencies any meaning?

I have calculated phonon frequencies of 2D sheet by analytical, classical solution as what we learned from basic solid state texts. But I have complex values for frequencies out of Gama point....

20 April 2018 4,131 6 View

In known group comparison/differences technique, what is the sample size for each group?

I need to use the known group differences to check the validity of the constructs as part of developing a new instrument. The instrument has seven first-order constructs and one second-order...

03 March 2018 4,179 2 View

Does Bio-Resonance really work?

Bioresonance is a non-invasive therapy which involves placing electrodes on your skin for diagnosis and treatment. The electrodes are connected to a Bicom machine which checks the energy...

24 February 2018 6,691 47 View

How can I make CD14 free ( getting rid of CD14 binding) after sorting Monocytes with FACS sorter? Any protocol for max yield and highest viability?

27 September 2016 7,644 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

What Model for Vaporization and Condensation in Fluent will be best?

Hello, I am running a simulation that includes water phase changes. These phase changes will take place below the boiling point of water so I need a method that can accurately model this. From my...

15 February 2021 4,973 3 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View

Post evaluation of close range photogrammetry experiments ?

I am performing a simple close range photogrammetry test and comparing outcomes of photogrammetry tools such as VisualSFM, Meshroom, Colmap etc. Kindly advice, what post evaluation tests/ criteria...

13 February 2021 6,410 3 View

How to calculate the Boiling point for metals at high vapor pressure?

In our experiment, some metal surfaces are exposed to radiation heat under high vapor gas pressure. I would like to ask if there is any way to calculate the boiling point for metals under high...

31 January 2021 249 4 View

How to remove BF3 etherate from a reaction mixture?

20 January 2021 6,322 2 View

Why isoelectric point (IEP) of alumina (Al2O3) dispersion decreases with the increasing of calcination temperatures?

We have prepared different alumina powders (fine/submicron-sized) from waste sources at different calcination temperatures (C.T.). From zeta potential-pH graph, we saw that IEP values decreased...

09 January 2021 9,404 3 View

Why do I keep obtaining the same secondary PCR band along with the specific product band?

Hello everyone! I need to amplify a specific DNA fragment in order to do Sanger sequencing for the detection of a point mutation in human patients. The fragment is in an exon of my gene of...

25 December 2020 1,805 3 View

How to overcome problems with introduction of modified DNA into C. glutamicum?

09 December 2020 7,715 5 View