Dear Matija,

the rising concentration of the in granulosa cells produced estradiol switches the cells producing kisspeptin. The kisspeptin influences both the negative and positive steroid feedback.

See

Skorupskaite K, George JT,  Anderson A. The kisspeptin-GnRH pathway in human reproductive health and disease. Human Reproduction Update, Vol.20, No.4 pp. 485–500, 2014 (free access).

Best regards

Pavel Travnik

Dear Pavel, thank you very much for your answer.

Ovarian steroids negatively regulation the kisspeptinergic neurons in the AVPV area of the hypothalamus in females. This negative regulation means that the kisspeptin release from the kisspeptinergic neurons is hindered/stopped. Kisspeptin in turn acts positively on the GnRH neurons in the hypothalamic and GnRH surge is maintained. GnRH inturn positively induce the pituitary gonadotroph cells to release LH and FSH. As the serum levels of LH n FSH increases so does the serum levels of estrogens. And high serum estrogen levels down regulated the GnRH neurons. Its a feedback loop. because the high serum levels of oestrogen goes down after sometime (because of the negative effect on kisspeptin neurons), the negative effect on kisspeptin neurons is reversed and the kisspeptin release is activated.                  Hope it help your query.  Cheers

Dear Shahzad, I appreciate your detailed answer. It is very helpful. Thank you.