Hi Chandan, your question is not very clear to me, btw I suggest you to have a look at this paper which can guide you through the right path.
Griffiths, B., A. Spilles, and M. Bonkowski. 2012. C:N:P stoichiometry and nutrient limitation of the soil microbial biomass in a grazed grassland site under experimental P limitation or excess. Ecological Processes 1:1-11.
Here are some papers on soil-nutrient studies, although they are not all specifically on C4 grasses and phosphorous, they should be helpful in developing your study:
Most of these papers use some kind of photosynthesis system to determine the effect of the nutrient. Taking readings from each treatment group with a gas exchange system can give you a lot of data including photosynthesis, transpiration, stomatal conductance as well as environmental readings. Here’s a link to a great handheld photosynthesis system that you might find helpful as you move forward with your research: