I'm planning to measure reactive oxygen species produced in tobacco cell lines under stress. Which method do you think is better? I see some articles measure ROI; it looks similar but I don't know if it's the same as ROS or not? If so, why they use a different name?
best,
Reactive oxygen intermediates (ROI) are successive 1-electron reduction products of O2 en route to the production of water. ROI include superoxide anion radical (O2−or O2•−), hydrogen peroxide (H2O2), and hydroxyl radical (•OH). Reactive oxygen species (ROS) include ROI plus ozone (O3) and singlet oxygen (O2) (whose production by cells is less clear). The terms ROI and ROS are sometimes used interchangeably.
You are planning to measure reactive oxygen species produced in tobacco cell lines under stress. Then, keep in this way and do not worry about "reactive oxygen intermediates" (ROI) that are, as mentioned above almost the same thing with subtle differences that have nothing to do with your aim. You have to look at the immense literature on this topic before you define the goal of your study: “why you like to study ROS produced in tobacco cells lines under stress” and do not care why they use different names. Reactive oxygen species (ROS) are chemically reactive molecules with unpaired electrons. There are superoxide anion O2.-, hydroxyl radical .OH, peroxide O2. -2 , or singlet oxygen 1O2. ROS are involved in many processes like carcinogenesis, cell proliferation, apoptosis, inflammation and cancer and in some regulatory mechanisms during hypoxia.That is what I can answer to your question.
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