What is the difference between positive control and spike system during a bio assay?
A positive control is a sample with naturally occuring (native) elevated amounts of the substance you want to measure (the analyte).
In a spiked sample you have added artificially an amount of analyte.
Sometimes you will not notice any difference but especially in protein-bound analytes like hormones you might notice that the analytical results are not similar for native elevated samples and spiked samples with the same concentration.
This can be caused by tight binding of the analyte to binding proteins in the native sample.
Hello, positive control mostly like qualitative test, just to find out presence of substances that you looking for. Spiking mostly used in quantitative tests it's just addition of known amount of analyte to desired matrix for calculation some validation parameters like recovery etc.
El control positivo es una muestra ya estandarizada y de la cual conoces todos sus componentes y características, ahora bien entonces en tu sistema de picos podrás comparar respecto a tu control positivo y conocerás que analitos o componentes o características tienes en cada pico de tu muestra a problema
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