Has anyone generated primary epithelial cells from Animal tissue?

21 February 2017 244 3 View

HPLC Agilent 1200 pressure problem when adding water to the mobile phase?

Hello, We have HPLC Agilent 1260 the problem is that the pressure bcom very low when we use the gradient mode (The addition of water during the time with Acetonitrile) causes this problem of pressure.

20 November 2016 5,539 7 View

How can I model the interaction between steel bars and concrete in ABAQUS?

I would like to know how can I simulate the interaction between steel bars and concrete such that can I determine the effect of steel bars specially in confinement. I do not know how to place the...

11 May 2015 3,376 4 View

I have a list of genes and I need to know which of them is a transcription factor. Can anyone suggest a tool?

I want to define a gene-gene network from microarray data using ARACNe tool. This tool needs to define transcription factors to generate more accurate results.

21 November 2012 2,136 26 View

Influence of (pulsed) electromagnetic fields on "microcirculation"?

I have recently come across to what looks like a classic homeopathic scam, namely the pulsed EM field miracle treatment as advertised by a German-based company called Bemer. As a...

04 July 2012 6,663 45 View

Is it misleading to report the average temperature of Earth?

The change in the average temperature of Earth is often used in the climate change discussions. However, temperature is an intensive thermodynamic variable, and thus it should not be averaged over...

30 March 2012 4,675 30 View

Help with culturing monolayer of human brain microvascular endothelial cells (hBMEC)?

I've been having a hard time forming an endothelial monolayer with good tight junctions. The person who trained me on the culture of endothelial cells worked mostly with mouse BMEC, and he used...

22 February 2021 8,253 1 View

Where can I get a Music therapy database for Alzheimer's Disease?

I am working on the effect of music therapy on persons with Alzheimer's Disease. I require EEG signals of healthy individuals and people with Alzheimer's Disease/Dementia that have undergone music...

22 January 2021 8,613 5 View

Is anyone aware of any freely available databases of normed works of art?

I am specifically interested in works of art for which ratings on multiple dimensions are readily available--for instance, ratings of beauty or of liking that were acquired from a sample of...

18 January 2021 296 5 View

Any known "open" tools for organizing information for research?

I am doing a bit of research on different areas (AI, cognition, music psychology, aging...) and have gathered so far a huge amount of information in the form of web links, pdfs, printed books and...

15 December 2020 8,004 5 View

How we can avoid or minimize problems of latency when making music in group through the web in real time.?

During the lock down and this pandemic one of the barriers that for music educators have become a dificulty has been the problems of latency in videochats, difficukting, even making unviable to...

07 November 2020 3,612 2 View

How to separate exosome and subcellular organelle (eg. ER) of mouse liver?

I want to do tissue subcellular fractionation of mouse liver. According to the method, the speed to separate ER is over 200,000g for 120 min (Song et al., 2006). However, liver exosome isolation...

12 October 2020 4,929 3 View

How to Design a Graph in PowerPoint Presentation?

Greetings, Recently I have been assigned several tasks of making presentations for webinar by a superior. The presentations are mainly about treatments in oncology such as immunotherapy and...

11 October 2020 9,593 4 View

How to syntheses Alkyd resin with bright colour from dark brown rubber seed oil?

All of the alkyd sample from rubber seed oil I have synthesized is always black in colour, such colour is not good for the production of white oil paint. I need help on what to do to achieve...

10 October 2020 9,860 1 View

Which study design and statistical test (MANOVA vs. ANOVA) to choose?

Hello academic community, I'm working on an experiment that tests the influence of music on software and I'm not sure, if I labeled the experimental design correct and secondly, which statistical...

24 September 2020 4,159 5 View

Is there a color theory or a practical method for the separation of colors into smaller dots as pointillists do?

Pointillists and divisionists separate colors into smaller dots and paints to blend in the viewer's eye at a distance. I wonder if it has already been formulated. I would very much like to know...

04 August 2020 5,875 3 View