it is a bit like the difference between salad and weed... :-)

it is a bit like the difference between salad and weed... :-)

For Peter Kapusta

As we all know, information from Wikipedia is intended for students and housewives. It is not recommended for scientists.

But if you are familiar with the scientific publication, where a rigorous scientific basis of this term - let me know.

Regards V. Mironov.

For Jan Voigt

I'm sorry, but I do not do agriculture, including cucumbers and cabbage, I am engaged in physics, in particular, the luminescence of the diamond crystals.

My question is related to the question of my colleague: How to decrease the autofluorescence of diamond prepared by CVD method?

Perhaps terms such as time taken from Wikipedia.

In this case, physicists say: how to reduce the influence of the substrate or the luminescence crystal diamond basics.

Do not litter the physics of medical and biological terms.

Sincerely Vasily

For Peter Kapusta

Dear Peter.

For more, please read my answer for Jan Voigt.

Our colleague asked:  https://www.researchgate.net/post/how_to_decrease_the_autofluorescence_of_diamond_prepared_by_CVD_method?_tpcectx=qa_overview_following&_trid=d7RhpYVRVVNcBzBYFLRMliK3_

 It's not my question.

The phrase "how to reduce the influence of the substrate or the luminescence crystal diamond" is not a question. This is my adaptation of our colleagues without the use of the term "autofluorescence".

My question is: what is the auto-fluorescence of the diamond? How is it different from the fluorescence of the diamond? Share a link on the scientific definition.

Incidentally, in my research I use (among others) Time-Resolved Confocal Fluorescence Microscopy method.

 Best wishes

Dear colleagues, thank you for your interest in my question.

About diamonds to tell me, there is no need.

About the autofluorescence:

Peter said: What Mahdi means there by autofluorescence is unclear and he should specify. From the phrasing of that question I infer he means simply the luminescence of the diamond material he gets.

With this statement I agree. As we try to reduce the glow of the diamond film, I replied to Mahdi.

Then Peter said: In biology, autofluorescence means the intrinsic fluorescence response of cells, tissue, etc., typically an unwanted contribution which is there without adding fluorescent probes or labels and interferes with the desired specific fluorescence signal.

It is well known that the biology, geology, and so on, wherever used physical methods of research, we must use the well-known physical terms, rather than invent new ones. Fluorescence - there is fluorescence in biology the same.

I support the statement Jan Voigt:  «it is a bit like the difference between salad and weed ..»

Really, the term autofluorescence - there is a weed from Wikipedia.

His question I draw attention to the correct use of terms only.

Russian saying: the flies - separately, burgers - separately.

Best regards.

Hello Vasily,

I have been looking an answer for the same question. As far as I have read and found, auto-fluorescence seems to be just another form of bio luminescence. The transitions resemble rather that of normal fluorescence, like between two singlet states. Hence the life times are shorter similar to normal fluorescence, of 10-100 ns. But I don't have any other explanation as to why it is called 'auto' fluorescence .

Dear Krishnakumar Chullipalliyalil

The lifetime of 10 - 100 ns is normal for ordinary fluorescence.

By all accounts, "auto" - fluorescence - is an unnecessary term from medicine or biology.

Imagine the experiment: together with the medicine, a phosphor is injected into the bloodstream to study the receipt of the drug for an organ. The receipt of the drug to the organ is visualized by the luminescence of the capillaries. In this case, organ tissues have their own luminescence, without a phosphor. It is this luminescence that biologists call autofluorescence.

Physicists usually use the terms "luminescence of the matrix (substrate)".

Borrowing of physical terms in other sciences is normal, but the use of bilological terms in physics is not correct.

Vasily.

Oh, thank you so much for the answer !

So it is just ordinary fluorescence from organic matrix, just that it kind of adds an extra noise to the fluorescence from the labels used. Such a confusing terminology !!

It makes no sense to use the same term for fluorescence from nano diamonds or any label of that sort. I had read papers on "auto-fluorescence from nano-diamonds", and have been wondering how would it be any different from its normal fluorescence. I completely agree with you, the use of biological terms in physics is utterly confusing !!!

It's a pleasure for me to help you.

Vasily