How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How post-hoc test should I perform for two way repeated measures ANOVA for 3 groups?

Hello everyone, I have 3 groups and 4 measurements so, I performed two-way repeated measures ANOVA. How can I detect the difference (which groups and which measurements) when there is significant...

27 October 2020 4,503 3 View

How do We Maintain Appropriate Cell microenvironment with 3D Cell Culture Method?

3D Cell Culture is newly optimized and promising discovery in cell organization research and other researches that needs evindences in cell culture (in vitro) level. Are 3D Cell Cultures mimic...

23 September 2020 7,109 4 View

How do We Clarify the Effects of Drugs on DNA Replication?

DNA Replication and its components are common targets in discovering new drugs as antimicrobial therapeutic agents especially while dealing with antibiotic-resistant species and other diseases...

21 September 2020 6,172 4 View

Can you give information about how to calculate the molarity of MEA?

Hi, I am trying to produce CZTS films by sol-gel spin technique. Can you give information about how to calculate the molarity of MEA? Did you calculate the MEA molarity separately for each metal...

11 July 2020 3,415 1 View

Do weak contribution equations within a module use the module's boundary condition in COMSOL?

I am trying to solve a partial wave equation for scattered quasi-TE waves. I found the COMSOL built-in Helmholtz equation module very close to my problem. The bottleneck was that, I couldn't...

03 June 2020 8,937 1 View

How Can I find a reverse primer with good rating, if I need to amplify entire gene?

I need to amplify the KCNA1 gene. I could not design the reverse primer with GC content ~50%, G/C content between 40-60%, rating 100, and no secondary structure. Any help much appreciated!

25 May 2020 7,276 9 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

How should I compare pre test and post test of three groups?

Hi everyone, I have 2 treatment groups and 1 control group. I measured some physical parameters as a pre-test and post-test. Should I compare 3 groups pre-test and post-test separately by using...

23 March 2020 8,599 8 View

Plasmonics as a collective oscillation of holes instead of electrons?

In metals, the outermost conduction electrons (electron plasma) interact with incident photons and according to the classical Drude model, they may resonate and build up longitudinal plasmonic...

17 January 2020 1,559 7 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View