What is the main advantage of using observer based fault tolerant controller(FTC) for sensor fault tolerance?

I am working on FTC for handling sensor faults in the system. I have read about observer-based FTC schemes which use error threshold-based logic between the states and estimated states. If a...

10 January 2021 5,771 2 View

Can a luenberger observer (even a modified version) be effective for a nonlinear affine system?

I want to know whether a luenberger observer or a modified form of it be effective for estimating the states of a system of the following forms: 1. x_dot = f(x,u,d); y = x 2. x_dot = f(x,u); y =...

20 December 2020 1,392 4 View

Implementing a non-local constraint in COMSOL Multiphysics?

I am trying to simulate a planar, high ohmic DC gas-discharge system. The model consists of a coupled set of equations, drift-diffusion for ions and electrons, and Poisson's equation in the gas...

07 December 2020 8,521 2 View

Is there any software for calculating hit bias (HB), missed precipitation (MP), and false precipitation (FP)?

I have a time series of two rainfall products. For error decomposition analysis, I have to calculate HB, MB and FB. Is there any software for calculating these variables.

27 November 2020 980 2 View

I'm looking for cell culture models for Alzheimer's by targeting amyloid beta peptide, tau protein, bace 1 enzyme ; can anyone give me details please?

I want to target particular protein/peptide/enzyme by cell culture methods. I would also like to know screening methods for cell cultures to analyse and diagnose the drug effect on particular...

24 November 2020 7,255 5 View

What modeling software can be used to analyse fate of pesticides using a fugacity multimedia approach?

Hello, I am trying to understand the fate of pesticides based on the fugacity multimedia approach. Is there software for such modelling? I know SWAT can be used for watershed-level pesticide...

15 October 2020 1,424 3 View

Can someone help me in designing off-MPPT mode in a Standalone DC Microgrid?

I am working on voltage control of a Standalone DC Microgrid consisting of Single PV source, a battery and loads. The PV is interfaced through Buck converter to the DC bus, the battery is...

01 October 2020 7,071 2 View

How to amplify extremely high GC-rich template?

I am cloning a whole cDNA of a particular gene and I have designed forward primer at start of 1st exon. The region downstream of forward primer is extremely high GC rich (around 85-89%). Because...

17 September 2020 6,571 11 View

How to apply load in abaqus ?

I have created a beam and i dont know, what is the maximum load it can take, Is there any way to increase the load continuously without giving a fixed value, so that at some load the beam will...

02 September 2020 6,246 9 View

I have a list of properties of tool steels here. Please let me know which of the below mentioned properties will change after heat treatment?

16 August 2020 730 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

How many ug should I choose as the protein amount when doing western blot in liver tissue?

25 January 2021 794 3 View