Should the RDE scan of electrochemical Oxygen Reduction Reaction be positive-going RDE scan or a negative-going RDE scan?

Hi, When we perform an electrochemical oxygen reduction reaction, should it be a positive-going RDE scan or a negative-going RDE scan? Thanks, Saurav

10 November 2019 4,707 8 View

What are the 3 basic conditions for impedance measurements that it must satisfy?

Hi All, Reviewer has asked to demonstrate that EIS data conforms the three basic conditions for impedance measurements. Can anyone please clarify what are these 3 conditions? Thanks, Saurav

04 May 2019 8,809 3 View

How to selectively block Selenium active sites?

Hi, I am working with noble-metal selenide for electrochemical hydrogen evolution reaction in acidic medium. Using DFT, I found that Se sites are active towards HER. I want to prove it...

08 September 2018 8,396 3 View

Can Mott-schottky plot be used in electrochemistry?

Hi, I have a semiconductor system that I am using to understand electroreduction reaction. Can I use Mott-schottky plot to comment on the n-type/p-type behavior of the system? My worry is that I...

02 March 2018 4,365 3 View

Can I use Stainless steel plate as counter electrode for accelerated degradation test in HER?

Hi, I have been using Pt coil as counter electrode for HER in 0.5 M H2SO4. Unfortunately, we can observe Pt leaching out from the coil during prolonged cycling. To avoid this I started using...

08 September 2017 6,375 7 View

Should we purge oxygen in KOH before performing electrochemical OER?

Hi, While going though the literature I found two sets of researchers- one who purge electrolyte with O2 before OER and another set who purge N2 before OER. I am confused what to do. Please...

08 September 2017 3,508 7 View

Can anyone help me with electrochemistry of Palladium in alkaline medium?

Hi, If I have two types of Pd in my system, will I observe splitted Pd-O reduction region in CV corresponding to the presence of two types of Pd? Thanks, Saurav

11 December 2016 5,078 2 View

Can I have an ECSA value of 10.9 cm2 for commercially available Pd/C?

02 March 2016 8,455 2 View

Can anyone please explain me the difference between NHE, RHE and SHE in a simple way?

I am too confused by the use of these terms (NHE, RHE and SHE) in research papers. Can anyone please help me out?

02 March 2016 8,755 5 View

Finding Problem with electron count calculation for ORR. Can anyone please help me out?

01 February 2016 5,871 7 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to quantify carbon deposition on CuZnAl catalyst?

Dear All, I want to quantify carbon deposition on the surface of the CuZnAl catalyst. I have tried thermogravimetric analysis, but I've got weight gain as the active Cu- get oxidised. Does anyone...

01 March 2021 1,907 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View