What is the best way to spike soil sample with Arsenic without too much change to the microbial population (and maintain its oxidation state) . The target concentration is 0 to 2,500 mg As/kg soil? Do I spike a small percentage and mix with uncontaminated soil, moisten the entire soil once (to field capacity), moisten at interval (let air dry each time) until the desire concentration is attained, or...?
The implication is that you want minimal toxicity. In that case you need to use As in the plus 5 oxidation state
the best way to spike soil with arsenic is to wet the soil at saturation percentage with arsenic salt solution with known amount of salt you want to add up and keep it at saturation percentage for 3 to 5 weeks and then allow it to come at field capacity, pulverize it to use in the experiment
Dear Osa
You can read these files.
Dear Osa
You can read this paper.
Assessment of arsenic toxicity in spiked soils and water solutions by the use of bioassays. Spanish Journal of Soil Science. DOI: 10.3232/SJSS.2012.V2.N3.05
Dear Osa,
I think that this publication can be very useful for your problem:
https://www.ec.gc.ca/faunescience-wildlifescience/1F2260C8-F7E4-4B4B-B36E-A902FEB917E0/RM42EnglishFinal%20-%20U.pdf
Hope it helps,
All the best,
Andrea
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