What is the best way to preserve fungal RNA in shipping (1 - 2 days)?

More Prashant Kishor Bhagwat's questions See All

What is the maximum value of Divesity Gian in MIMO? For two antenna system its 10dB. As no. of antennna increse diversity gain increase or decrease?

Somewhere on the internet, it's reported the maximum diversity gain limit theoretically can be achieved is 10dB. Some papers said it increases with more antennae and some said it will decrease.

09 June 2024 5,121 2 View

Fully funded phd ?

any fully funded phd position in wildlife ?

25 May 2024 7,976 2 View

Access to document containing CPCB calibration curve and uncertainty for PM2.5, SO2, O3, and NO2 monitors?

I am wondering if anyone has come across calibration curve and uncertainty for PM2.5, SO2, O3, and NO2 monitors maintained and operated by the CPCB? Please share link herein for the same if you...

25 April 2024 7,164 5 View

How to model or program a cascade refrigeration system using natural refrigerants on MATLAB ?

What are different ways of modelling and simulation of cascade refrigeration cycleusing natural refrigerants on MATLAB without using simscape ? How to write program for it and connect MATLAB...

02 April 2024 487 2 View

Classical text is important or research journals are important?

because people are citing/talking less about Classical texts discovered by Rishi/sages. Rishi has the broad scientific knowledge as per their practical and theoretical practices.

30 March 2024 2,013 0 View

How to interpret the LCMS data with using peak values?

How to evaluate the compounds with usage of peaks and their respective values in LCMS - TOF instrumentation? Is there any web portals for the data interpretation? I Have got Both LC (2 Files) MS...

13 March 2024 7,940 1 View

Understanding factors leading shift from spiritual-cultural drinking to modern alcohol consupmtion in Warli tribe?

Alcohol consumption shift. Socio-econo-cultural factors, globalisation, westernisation, religious conversion role in alcohol consumption shift

12 September 2023 4,757 0 View

How fin and fan design is done for for thermoelectric refrigeration system ?

I have not understood here the method of design of fin and Fan for thermoelectric refrigeration system in paper attached .....................

09 August 2023 8,355 0 View

What best method to obtain correct composition of Al2024 series ? OES or EPMA?Steps?

I have tried OES method to obtain composition of 2024 T4 multiple times. As per standard composition Cu should vary between 3.8-4.9 and Mg should vary between 1.2-1.9. The problem coming is that...

18 July 2023 450 0 View

Are D16AT and 2024-T4 same ??

In all research paper i found the Aluminium alloy D16AT and 2024T4 having same chemical composition . They are subjected to same heat treatment process. Are they different or same ???

16 July 2023 8,039 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View