Carolina Cuevas Mendoza

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Questions related from Carolina Cuevas Mendoza

What is the best way to precipitate protein after ultracentrifugation in Optiprep gradient?

Hi, I want to extract lipid rafts by using an Optiprep gradient in order to analyze some receptors. After ultracentrifugation and recovery of the fractions, I used TCA (trichloroacetic acid) to...

19 July 2023 292 0 View

How do you calculate the methyl-β-cyclodextrin concentration to use when depleting cholesterol?

I'm using methyl-β-cyclodextrin (MBCD) to deplete cholesterol from mammalian cells. I'm following the Mahammad and Parmyrd protocol, in which cells are treated with different MBCD concentrations,...

16 November 2021 9,020 1 View