I'm working in production and optimization of pigmented fungi biomass (Gibberella/Monascus) in order to produce an alternative biocolorant. So what should I do to make it grow well?
You can adjust the C:N ratio ( ( what agrowaste are you using) ; moisture content, add yeast extract for growth factors; inoculum age and size are among the few parameters that you can optimse for pigment production
I fill particle size of your substrate will also be an important factor. Addition of amino acid rich macroalgae will help u to get maximum pigment production.
Do determine the C:N ratio and add nitrogen supplement accordingly. Check my page at RGate to get a copy of my paper as guide. Particle size as mentioned by Thiyam G is also important. Not too fine ( can cause compaction if moisture content is high) - 2-5mm size will increase surface area for fungi to colonise rapidly. Aeration via mixing maybe needed if you are doing large volumes. Are you planning a tray system?.
Hai Afif you can check this paper in my profile hope it might be useful. Fungal utilization of a known and safe macroalga for pigment production using solid-state fermentation
Pay proper attention to mixing and ventilation of the SSF chamber.Keep he particle size that helps easy and thorough turing of microbial mass on the substrate and porosity of the bed. Contamonation due to bacteria will not allow healthy growth of fungus and the pigment formation will be affected.
You can use the methodology described in: Mendes G et al., 2015. Optimization of Aspergillus niger rock phosphate solubilization in solid-state fermentation and use of the resulting product as a P fertilizer. Microbial Biotechnology,
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