What are the most up-to-date methods for defining/mapping global land degradation and restoration?

I am seeking the best current methods and datasets (highest possible resolution) for defining and assessing global land degradation - ideally with a time series. I know there are different ways of...

24 November 2020 6,013 5 View

How do I write up thematic analysis results concisely?

Hello everyone, I've been writing up thematic analyses of interviews for the first time ever, and I can't help but write way too much for a thesis, let alone the papers that will make up a...

24 February 2020 5,044 11 View

Help With Autodock Vina error?

Hello, I've set up docking for vina and when i try to run the .txt file containing the inputs for docking i get this result: Parse error on line 7877 in file "protein.pdbqt": Unknown or...

28 January 2020 2,394 6 View

Would anyone working in animal and wildlife research be open to answering a few questions I have regarding how you got to where you are in your field?

Hello, I am a current BSc student in Zoo Biology working towards a career in animal and wildlife research in the field. As apart of my course I am hoping to interview or get into contact with...

11 October 2018 3,710 3 View

How to compare two experimental data sets with only a few overlapping individuals?

I have two experiments from the last two years in the field. The experiments are supposed to be the same, but different environmental conditions slightly skewed the exposed conditions relative to...

16 October 2017 9,864 3 View

Generating cDNA from low yield RNA?

25 April 2017 1,040 3 View

Labelling monoamines with dansyl chloride?

Hi, I'm trying to observe where dopamine localises at the cell surface of my cells of interest but fluorescent dopamine is very expensive to buy. I've read online that I should be able to label...

12 October 2016 5,404 2 View

GFP expression in cell-free systems - Why dasherGFP can be expressed just fine in a cell-free system, while cometGFP doesn't seem to work?

Can anyone think of a potential reason why dasherGFP can be expressed just fine in a cell-free system, while cometGFP doesn't seem to work? I'm not sure if it could be a translation problem or a...

22 February 2016 2,231 4 View

Programs to identify specific Protein-DNA interactions?

Are there any programs out there that can identify which specific DNA motif a transcription factor binds to, given the entire promoter sequence and TF sequence/structure. I've got the promoter...

12 February 2016 2,630 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View