What is the best way to do structural connectivity analysis? Immunofluorescence of radiological imaging?

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Which catridge and solvents can i use to extract ARV drugs in wastewater using the SPE method?

I am doing a research aimed at removing ARV drugs from wastewater. I intend to use SPE for extraction and then analyse the treated samples with LC/MS. I am currently trying to identify the most...

02 July 2019 5,203 3 View

Why is there still a few females studying physics?

Currrently working on this paper and would like to know views and thoughts of scholars?.

11 December 2018 2,850 3 View

In TRFLP, why do people use two labeled primers while others only employ one?

I am doing studies on plant endophyte diversity using TRFLP as the main tool. Why do some papers use two labeled primers (for forward and reverse; ex. 799f labeled with 6FAM and 1492rh labeled...

16 July 2015 727 13 View

What staining protocol is the best for staining for cell death in the rodent brain?

Is it Giemsa staining?

16 April 2013 4,080 2 View

What is the best way to raise children?

Important information to help parents with trouble some kids.

01 January 1970 4,335 12 View

Is Voting Still Relevant or we need to start thinking of other way of electing Leaders as it seems to be disputed and manipulated all the time.

Thinking of ways to reduce corruption in the elections systems.

01 January 1970 4,567 12 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

High aspect ratio hexahedral mesh optimization solver?

I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...

26 February 2021 7,279 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

Anyone with some Transgenomic Wavemaker software? Seeking help from the chromatography community?

I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...

23 February 2021 9,181 1 View

How to validate regridding results of environemental data to higher spatial resolution in R?

I am working with several continous, environmental marine datasets at varying original spatial resolutions. My aim is to regrid each dataset to one common, high resolution grid. I am currently...

22 February 2021 2,290 2 View