Is there some specific drug to induce injury only in muscle fibers without affecting peripheral nerves?

I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...

30 October 2020 9,077 4 View

How do I extract data Hazard Ratio and CI from Kaplan-Meier curves?

I am conducting a meta-analysis of Hazard Ratios (HR), however, most of the studies presented only Kaplan Meier curves without the HR and Confidence Intervals (CI). I am extracting data manually...

23 June 2020 6,584 1 View

How can I solubilize HgNO3 in water?

I need to solubilize HgNO3 in water at different concentrations, but I have notice an orange precipitate formation when I dissolved the salt in water. Probably it is mercury oxide. How can I...

28 March 2019 1,277 3 View

Procedure for thawing cryopreserved hpc sample for flow cytometry viability via 7-aad?

Currently our lab performs post thaw viability via trypan blue. We would like to begin using flow cytometry 7-aad for our post thaw viability. Our attempts so far at thawing our samples have led...

22 September 2018 5,396 3 View

Analysing questionnaire with multiple constructs in SPSS

27 May 2017 9,677 5 View

How to account for phylogenetics in variables (phenols, nitrogen, leaf toughness etc) for australian plant families? PGLS? where do you find the data?

I have a data set of chemical and physical traits (and herbivory data) from trees and vines at one of my study sites. Unfortunately, there was no overlap in the families trees vs vines came from...

26 February 2017 3,263 3 View

It is possible to detect (by WB) a membrane receptor, mainly in the cytosolic fraction after a differential centrifugation?

07 December 2015 5,342 3 View

Aside from looking to prototypicality studies for category lists, are there more sophisticated and open-source databases available (i.e. animals)?

I am looking to generate culturally unbiased and medium frequency words to be used in a memory task.

23 March 2015 4,056 3 View

Has anyone detected BDNF in tissue homogenates by ELISA?

08 January 2015 3,081 7 View

What is the effect of Arginine on siRNA binding?

23 July 2014 363 0 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

Must a control group to be in the same semester that a experimental group for a study in education?

Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...

22 February 2021 3,653 11 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Can I arbitrarily take a cycle number of 40 for the gene that was not expressed at all in qPCR results calculation?

I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...

20 February 2021 6,243 10 View

How to overcome convergence issues in concrete arch models?

Hi, I have modelled a three-span arch bridge with soil fill over along with pedestals. The FE modelling is done in LUSAS with HX8M solid elements (i.e. 3D solid modelling). The non-linearity is...

20 February 2021 1,346 2 View