I'm conducting a phytoremediation experiment on pesticides such as urea and 2,4 DCP from water, so I need suggestions on the best way to determine the concentration of these pesticides in the plant?
This is a typical selectivity + sensitivity problem. About 50 years ago C.S.Giam, (my PhD advisor). R.C Hall (a fellow graduate student) and I developed and reported a method using a pyrolysis reactor on the front of the GC fitted with an electron capture detector. We were detecting below ppb levels using this strategy on similar materials extracted directly from soil. This was reported in about 1969 in Journal of Chromatography.
@Scot Actually, I saw a company at the ASMS 2019 meeting who was selling a unit that you put the ground solid sample in an aluminum foil and put in a special chamber and was heated with a special unit to vaporize any volatile compounds from the sample directly to the GC oven set at a relatively low temperature (30-40 C) to freeze all of the analytes at the head of the column. The analytes were heated with the temperature programming as usual. If you need the name of this company, contact me and I will dig the flyer I got from the conference.
Thank you Dr Chamkasem for the kind offer. I did the work at Texas A&M where I did my PhD (1971). Subsequently I have been involved in developing instrumentation and applications for the last 48 years. (Hplc, GPC, Rheometry, viscometry, mechanical testers, software, data systems, system controllers,
etc.).
Enyoh Christian Ebere, probably your best option is LC-MS/MS (triple quadrupole if you want a quantitative result). It is also important to use a sample preparation technique (liquid liquid extraction or solid phase extraction) prior to the analysis. Then, you can do a clean up and a pre-concentration at the same time.
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