Dear RG community! When performing rhizosphere competence assays with entomopathogenic fungi (EPF) I usually applied the conidia onto plant seeds using carboxymethyl cellulose in order to really glue them onto seeds. But the process is messy and time consuming. Is there a faster/ easier way to apply conidia onto seeds? Recently it has come to my attention that a simple immersion of seeds in the conidial suspension results in the conidia to become (irreversibly?) attached to the seeds. Would this work?
Thanks in advance for your suggestions and help!
You may prepare conidial suspension in Jaggary (Gur) solution (1%) and immerse the seed for 5-10 minutes.
Dear Jaka,
Carbixymethyl cellulose is one of the best materials for seed coating. Gum Arabic is also used for the same purpose, the difference is the Gum Arabic is natural material. For the simple immersion technique, I can definitely tell you that it is working as well as the other techniques mentioned above. The difference is the amount of conidia that will stick in the seeds. I recommend you to increase the concentration of conidia in the solution applied for seed coating. Immerse the seeds in the conidia solution for 24 hours and then let them dry under laminar hood.
I hope this recommendations will help you.
Good luck
Dear Badreddine Sijilmassi, if I understood you correctly, you would recommend soaking the seeds in a 'dense' conidial suspension, without any 'glue' for 24 h? But wouldn't the conidia settle on the bottom of the coating vessel? Should I orbitally shake the seeds during the coating procedure, or not? Thanks!
Dear Jaka,
Exactly. You should soak them under shaking (120-150rpm) with the appropriate temperature for your conidia species.
Hi Jaka
you can check this reference may be useful for you
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=2ahUKEwiSjqjyq8jfAhWMpIsKHSTCDpAQFjACegQIBxAB&url=https%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%2Fjournal.pone.0185903&usg=AOvVaw0jgJYq9Y2mv6R0NfXmk2W-
Ref.
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=2ahUKEwiSjqjyq8jfAhWMpIsKHSTCDpAQFjADegQIBhAB&url=https%3A%2F%2Fwww.tandfonline.com%2Fdoi%2Fpdf%2F10.1080%2F09583150903545035&usg=AOvVaw2V00TofJgoKJ26RCPi-D5t
Ref.3
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&cad=rja&uact=8&ved=2ahUKEwiSjqjyq8jfAhWMpIsKHSTCDpAQFjAFegQIBBAB&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4535486%2F&usg=AOvVaw0PCv3EZ0a2c0LRcWXYGw0J
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