A very interesting question and I look forward to feedback by other colleagues. Here is a comprehensive review article on SIGS: Spray-induced gene silencing: an innovative strategy for plant trait improvement and disease control by Ramesh R. Vetukuri et al. I hrope Dr. Ramesh Vetukuri is on Researchgate to address your question. Best regards.. Cheers.. Simon

Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake

Lulu Qiao, Chi Lan, Luca Capriotti, Audrey Ah-Fong, Jonatan Nino Sanchez, Rachael Hamby, Jens Heller, Hongwei Zhao, N. Louise Glass, Howard S. Judelson, Bruno Mezzetti, Dongdong Niu, Hailing Jin

First published: 27 March 2021

https://doi.org/10.1111/pbi.13589

Simon Francis Shamoun Simon, thank you! I see a significant gap between siRNA-induced gene silencing in model experiments and practical results on plants under the infection + natural stresses. Even in the most successful experiments there are a lot of facts that cannot be explained (unexpected interaction between several RNAs, cultivar difference, treatment timing difference, temperature, plant age effect, etc.).

Alex Ignatov Dear Alex, you raised very important issues indeed. Thank you. Cheers.. Simon

Hi Alex

I think this paper is useful for you about the exogenous application of double-stranded RNA

"RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges"

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwjZr-iCs5ryAhV6hP0HHa-LAfcQFnoECAkQAw&url=https%3A%2F%2Fwww.mdpi.com%2F2223-7747%2F10%2F4%2F650%2Fpdf&usg=AOvVaw0mPqFcuAoXvl8PvkZ05yrW