Hi. I'd like to know if a NH2-bearing molecule (such as antibody) can easily bind to a surface with succinic anhydride functional group or it firstly needs EDC to activate this functional group before the binding?
Thanks for your time.
If you really have a succinic anhydride functional group, you do not need any activation. However, anhydrides hydrolyze very easily in water or ambient air, and are subsequently inactive.
Thanks for your answer Michael.
I'd like to functionalize my microparticles' surface with 3-(TRIETHOXYSILYL)PROPYLSUCCINIC ANHYDRIDE (TESPSA). I found that two groups have binded antibody/cell to the TESPSA-functionalized surface/particles.
Following is the papers:
Gang, A., et al. "A simple two-step silane-based (bio-) receptor molecule immobilization without additional binding site passivation." RSC Advances5.45 (2015): 35631-35634.
Zhang, Xingdi, et al. "Functionalized mesoporous bioactive glass scaffolds for enhanced bone tissue regeneration." Scientific reports 6 (2016): 19361.
I couldn't find any information about the activation of succinic anhydride group with EDC in these two papers. But, Gang et al. dehydrated (cured and annealed) the functionalized surafce prior to immobolization of antibody at 120 degree c for 1.5 hr.
Do you think if dehydration is a good choice instead of activation of succinic anhyride group with EDC to immobilize my particles with a NH2-bearing molecule?
TESPSA is a very uncommon reagent. I do not see a reason, why you should use it. If you want to immobilize a protein, there are much simpler and proven methods, e.g. based on amino- or epoxy-silanes.
Thanks very much for the introduction of epoxysilanes. I didn’t know about them.
In fact, I was looking for safer chemicals to work with and I found TESPSA. Besides, it seemed to me that they should be easy to work with. But, I had this question if they need activation or not.
I do not think that TESPSA is safer than other silanes. It is definitely more difficult to work with due to its extreme sensitivity to hydrolysis.
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