I want to extract and quantify EPS (Exopolysaccharide) from bacteria (originated from plants).
I precipitated EPS using 3 volumes of cold absolute EtOH from cell supernatant. Then I washed precipitates twice with cold absolute EtOH.
Then I tried two ways as follows:
1) Dissolve EPS in distilled water directly after washing
2) Dry EPS then dissolve it in distilled water
However, these precipitates were not dissolved completely in both ways thus I cannot measure the exact amounts of EPS. (I can see some floating particles)
There is any proper way for dissolving EPS in distilled water?
Seorin Jeong
Also, what is the difference in unit of EPS measurement (mg/l, mg EPS/mg protein)?
Is there any advantages in mg/mg protein?
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