I intend to carry out PCR on extracted DNA from a bacteria and human samples. I want to know the proper primers to use, buffer,dNTPs, Taq polymerase. And if its proper to use Magnessium chloride and DMSO.
It depends upon what kind of organisms are you talking about. If you are willing to amplify the 16S rRNA REGIONS OF BACTERIA OR 18 s OF FUNGI then you should select any universal primers from the company like Macrogen. In order to amplify the human gene region you have to be more specific about which region you would like to amplify and select the primer then only. I generally use their 518f and 800R primer in case of bacteria.
I have provided the link to the available primers that you can order and use
https://www.macrogenusa.com/support/seq/primer.jsp
Also you are asking the protocols with detail ingredients to amplify the regions, there are lots of protocols but you can try the one that i have done and you can download my paper from research gate to see the full protocol for amplification.
I hope this will help
Most of the information you need will be in a protocol given with your taq polymerase.
Mostly RAPD DNA primers can be used for all organisms DNA. It is used randmly for amplification of DNA. after amplification sequence can be determined of that amplified gene / locus. It is better if you are want to amplfication randmly not for specific gene.
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