I have tried PEI and lipofectamine 2000 to deliver a GFP plasmid into HEp-2 cells, both showed low transfection efficiency. Does anyone have good experiences in HEp-2 transfection?
Lipofectamine 3000 reagent certainly worth trying if you're facing low transfection efficiency with HEp-2 cells. Always perform control experiments and assess cell viability and transfection efficiency to ensure that the new reagent is working effectively for your specific application. Additionally, consider running parallel experiments with your previous reagents for comparison.
Transfection can be a trial-and-error process, and the right reagent and conditions can vary depending on the cell type and the specific plasmid being used.