He Huang

3 Questions 4 Answers 0 Followers

Questions related from He Huang

What is the best transfection reagent for HEp-2 cells?

I have tried PEI and lipofectamine 2000 to deliver a GFP plasmid into HEp-2 cells, both showed low transfection efficiency. Does anyone have good experiences in HEp-2 transfection?

20 September 2023 4,673 1 View

Does anyone have experiences with Immuno-Precipitation of ApoB?

ApoB is large protein, ~550KDa. I searched the literature and most of the published articles brifely described the prosedure of IP. They added SDS in lysis buffer and lyzed the cells overnight to...

19 November 2014 6,447 2 View

Where does lentiviral shRNA insert into the genome?

Can the insertion of shRNA into host cell genome influence certain gene expression? How can I know the inserted site of lentiviral shRNA in a stably transduced cell clone?

04 November 2013 8,733 6 View