What is the best tool to quantify H2AX foci?

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Bruno Vaz

Would recomend imageJ. http://microscopy.duke.edu/HOWTO/countfoci.html

José F Ruiz

People usually use Metamorph software

See example in Murga et al JCB 2007 (Fig.3) and Methods (The quantification of H2AX intensity per focus was performed with software (Metamorph 7.0; Molecular Devices).

Vered Domankevich

Thank you for your answers

Metamorph seems like a very good software, however I seek for free software

Any suggestions?