Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Shall we split the data into 60% of training and 40% of testing. And apply k fold cross validation for training and the best trained model for test?

Is the above method of splitting the data set into taring and testing, and then apply k fold cross validation for training, and then the trained model to classify the test set is right?

04 April 2020 9,040 3 View

Scalogram for huge data?

I have a huge data for which I want to apply wavelet and plot scalogram. the below code works for small data. However, I have a huge signal, for which I am not able to plot scalogram. Can anyone...

10 April 2019 1,959 3 View

How to do miRNA isolation from hematopoietic stem cell with good purity?

Hi, currently I am isolating miRNA from hematopoietic stem cell using miRNeasy Qiagen kit. However, I didnt get good purity for both A260/280 and A260/230 plus low concentration as well. According...

14 October 2018 6,123 1 View

What are the causes for low Free Fatty Acids (0.16) in Cocoa mass?

Free fatty acids content should not be more than 1.75% in cocoa mass according to FSSAI standards and got 0.16 % as result. I want to know the reason behind occurrence of low free fatty acids in...

22 November 2017 9,993 4 View

How to concentrate DNA? I lost 70% of DNA while concentrating it using 3M sodium acetate and 2 volumes of ethanol? Is there any other alternative?

Read 7 answers by scientists to the question asked by Ramya Murugesan on Oct 6, 2016

05 October 2016 9,702 6 View

Does Lysozyme cause the lysis of cell wall as well as inner membrane in gram positive bacteria?

08 May 2016 289 14 View

Does J774A.1 form clumps in cell culture flask?

I see the newly thawed J774A.1 cells form clumps of about 10-20 cells. On sub-culturing the size of the clump reduced to about 5-10. Is that normal? I am using DMEM media supplemented with 10%FBS...

10 January 2016 2,226 5 View

Are there any books for image processing techniques using verilog?

Are there any books for image processing techniques using HDL verilog?

05 January 2016 7,344 2 View

Number of samples for discovery based study by NGS?

Hi all, To conduct a cancer based discovery study by NGS what is the optimal number of samples [minimum number to be precise] which would yield convincing results as it is difficult to sponsor for...

12 November 2015 5,926 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

Treating Intermediate Variable in Linear Regression Analysis in SPSS?

While performing the Linear regression analysis in SPSS version 26.0.0. my intermediate/mediation variable goes to the excluded section. Please share your valuable suggestion "How to restrain the...

25 February 2021 4,591 5 View

Cox regression baseline hazard?

Dear colleagues, I would like to make a mathematical equation of Cox regression model, however I am stuck with baseline hazard. I clearly understand interpretation of coefficients, but I can't...

24 February 2021 6,207 3 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View