I am planning to induce antiangiogenesis on CAM assay using ethanol, benzene, and acetone. What will produce the best yield?
That really depends on the active compound in a particular plant extract. If a plant produces a non-polar compound, the benzene or acetone may be the best. If the active compound produced by a plant is polar, ethanol or even water is best.
Some people extract the plant in several solvents going from less polar to more polar. As an example hexane -> ethyl acetate -> methanol -> water. Other people extract in ethanol since it can extract non-polar and polar compounds.
I like to use methanol or ethanol and run a column screen which provides a pathway to purify the active compound(s) .
http://www.teledyneisco.com/Documents/Liquid%20Chromatography/Posters/New%20Techniques%20in%20Extract%20Column%20Screening%20Poster.pdf#search=column%20screening
http://www.teledyneisco.com/Documents/Liquid%20Chromatography/Posters/New%20Techniques%20in%20Extract%20Column%20Screening%20II%20Poster.pdf#search=column%20screening
Links updated 14 Jan 17
If you want activity in crude extracts, go for methanol or aqueous solvent for extraction.
Hi
I think you should follow bioactivity guided extraction and fractionation. Firstly the powder will be subjected successive extraction with different solvents in increasing order. All the extracts will be screened for activity. The active fraction may be subjected to column or HPLC to detect active phytoconstituents.
Good luck.
Can you avoid the benzene? Some people have gotten really sick.
Toluene has been recommended as a replacement that's somewhat less toxic although it still smells bad. Some labs won't even allow benzene. Good luck!
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Pharmaceutical Botany
- Plant Extracts