I am trying to do a TLC on water soluble extracts from some plants
Dear
The use of solvent depends on the nature of compounds expected to be excited in the extract and its polarity. Aqueous extract containing the polar compounds, so you need to use a polar solvent e.g. methanol, ethanol. However for TLC analysis you need to check the the solubility of your target compounds in the chosen solvent.
Good luck
Why do not use water as solvent if the sample is water soluble?
Have you any particular reason?
Agree to Alsidig, You can try Methanol or Ethanol but your extract will not dissolve fully in it.
I agree with Erkan halay first you should try to solubilise whole constituent that may be possible in mixture of solvent like ethanol-water, methanol-water, or acetone- water. Water should be in minimum amount.
I think you should try with methanol with little portion of water, how much water?. Using the minimum necessary to dissolve all the extract , it means you should use methanol and add water drop by drop until the extract is dissolved. Of course you should try to use only some milligrams of your dry extract .
- Badges
- Science topic
- Similar topics
- Biological Science
- Plant Sciences
- Ethnobotany
- Natural Product Pharmacology