It depends on a number of factors including cell-type and usage of exosomal preparation after isolation/purification. Assuming that you would like to use exosomes for characterization of their "cargo" (proteins, RNA, and DNA), ultra-centrifugation may be a preferred method. However, the other method may give you more starting material.
Thank you professor, and my question is really focused on the work with salivary fluid which is a small sample size (200 uL aprox), so the kit is a good approximation to start.
ultracentrifugation is a good old school approach. but it takes a while to bring it to perfection (esp with sucrose gradient) and also its for low throughput applications. Total exosome isolation reagents (Invitrogen) works really well for exosome precipitation
As previously mentioned, your isolation method depends on your downstream application and your starting material. With precious starting volumes where high yield is required, ExoQuick-TC (SBI) is probably your best bet. Ultracentrifugation can be time consuming and result in low yields of exosomes.
With your starting material (saliva) we have had successful results isolating exosomes with ExoQuick-TC that were further validated with NTA and Western blot.
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