I want to determine the CUPRAC antioxidant assay for a plant material extracts from different solvents. There are two methods to use blank in spectrophotometric way-either reagent blank or solvent blank. Which will be best?
The best procedure for using a blank in CUPRAC assays is to use a reagent blank. This involves measuring the absorbance of the reagents without the addition of a sample. This is more accurate than using a solvent blank, which involves measuring the absorbance of a solvent without the addition of a sample. The solvent blank may not be a good indicator of the actual absorbance of the sample, as there may be small amounts of impurities present in the solvent.