Hi,

I'm measuring cellular Ca-3 activity with a fluorescence kit (EnzChek Caspase-3 Assay Kit 1) using a Synergy H1 microplate reader.

With this current fluorescent kit the final volume to measure is 100 uL in a well from a 96 well plate.

When measuring the fluorescence I have to fix the gain (fine) but I need the probe height.

What is the best probe height for this sample volume?

Thanks

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